UM-SCC-129

UM-SCC-129 is a human head and neck squamous cell carcinoma (HNSCC) cell line established from a patient tumor at the University of Michigan Head and Neck Oncology Laboratory. As part of the UM-SCC cell line panel — one of the most extensively characterized and widely distributed HNSCC cell line collections in the world — UM-SCC-129 provides a defined in vitro model for head and neck squamous cell carcinoma research. The UM-SCC series was developed over 40+ years from over 100 patient donors at the University of Michigan and is distinguished by well-documented STR profiles, TP53 mutation status, and radiation sensitivity data across the collection.

UM-SCC-129 is applicable in HNSCC biology research, radiation sensitivity studies, chemotherapy sensitivity testing (cisplatin, 5-FU, cetuximab), EGFR pathway analysis, TP53 functional studies, and evaluation of novel targeted agents. The UM-SCC panel culture is well-established in DMEM with 10% FBS and 1% NEAA, matching standard UM-SCC culture conditions across the collection. As with all UM-SCC lines, STR authentication and TP53 mutation status confirmation are recommended prior to experiments.

37°C, 5% CO₂, humidified atmosphere.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 200 x g for 5 minutes, carefully discard the supernatant containing freezing medium.

7. Follow the procedure described under Post-Thaw Recovery

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.