CHO-FCGR2B

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CHO-FCGR2B cells are recombinant Chinese hamster ovary (CHO) cells engineered to stably express human Fc gamma receptor IIB (FcγRIIB; FCGR2B/CD32B), a low-affinity inhibitory receptor for the Fc region of immunoglobulin G (IgG). FcγRIIB is broadly expressed on B cells, dendritic cells, monocytes, macrophages, and other immune cell populations, where it functions as a critical negative regulator of immune activation. Upon co-engagement with activating receptors, FcγRIIB recruits phosphatases through its immunoreceptor tyrosine-based inhibitory motif (ITIM), thereby suppressing downstream signaling pathways involved in antibody-mediated immune responses. Dysregulation of FcγRIIB signaling has been implicated in autoimmune disease, chronic inflammation, and altered responses to antibody therapeutics.

CHO-FCGR2B cells are widely used in therapeutic antibody development and immunology research to evaluate Fc-mediated interactions, receptor selectivity, and inhibitory signaling mechanisms. These cells support quantitative assessment of IgG subclass binding, Fc engineering strategies, immune complex interactions, and antibody-dependent modulation of Fcγ receptor pathways. They are particularly valuable for screening monoclonal antibodies, bispecific antibodies, Fc-fusion proteins, and glycoengineered biologics designed to alter FcγRIIB engagement. CHO-FCGR2B models are also frequently applied in flow cytometry assays, receptor occupancy studies, reporter assays, and high-throughput screening platforms intended to characterize Fc receptor specificity and functional activity.

For adherent cultures: DMEM:Ham's F12 (1:1), w: 3.1 g/L Glucose, w: 2.5 mM L-Glutamine, w: 15 mM HEPES, w: 0.5 mM Sodium pyruvate, w: 1.2 g/L NaHCO3 (Cytion article number 820400a)

For suspension cultures: CHO Growth Medium A (from InSCREENeX; InSCREENeX catalog number INS-ME-1039)

37°C, 5% CO₂, humidified atmosphere.

After thawing, split the cells at a ratio of 1:2 to 1:3 in T25 flasks and allow the cells to recover from the freezing process and to adhere (for adherent cultures) for at least 24 hours.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.

To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.