Panc02-Luc

Panc02-Luc is a luciferase-expressing derivative of the Panc02 murine pancreatic adenocarcinoma cell line. Panc02 cells originate from chemically induced pancreatic ductal adenocarcinoma in mice and are widely used as a syngeneic model of pancreatic cancer in immunocompetent murine hosts. The introduction of a luciferase reporter enables highly sensitive bioluminescent imaging of tumor cells in vitro and in vivo, facilitating noninvasive longitudinal monitoring of tumor growth, metastatic dissemination, and therapeutic response. These properties make Panc02-Luc a valuable platform for pancreatic cancer biology, immuno-oncology, and preclinical drug development studies.

Panc02-Luc cells are commonly utilized in orthotopic and subcutaneous mouse tumor models to investigate tumor progression, stromal interactions, immune cell infiltration, and mechanisms of resistance to chemotherapy or immunotherapy. Because Panc02 tumors can be established in syngeneic mouse strains with an intact immune system, the model is particularly useful for evaluating checkpoint inhibitors, adoptive cell therapies, cancer vaccines, and combination treatment strategies. Luciferase-based imaging enables repeated quantitative assessment of tumor burden in living animals, reducing experimental variability and supporting real-time evaluation of treatment efficacy.

Panc02-Luc cells are used for studies of pancreatic tumor cell proliferation, migration, invasion, cytokine signaling, metabolic adaptation, and apoptosis. The biological behavior of the model may vary depending on the luciferase construct, promoter system, and clonal selection strategy used during engineering. Further characterization data, including reporter stability, luminescence intensity, and metastatic potential, may be important for specialized experimental applications.

37°C, 5% CO₂, humidified atmosphere.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 200 x g for 5 minutes, carefully discard the supernatant containing freezing medium.

7. Follow the procedure described under Post-Thaw Recovery

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.