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Panc02-Luc
Tissue
胰臟
Disease
小鼠胰管腺癌
Synonyms
Luciferase Reporter Panc02 Cell Line

Documents

Panc02-Luc

Cell line · Product number 305706

Panc02-Luc is a luciferase-expressing derivative of the Panc02 murine pancreatic adenocarcinoma cell line. Panc02 cells originate from chemically induced ...

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01General information

Description

Panc02-Luc 是 Panc02 小鼠胰腺腺癌細胞系的螢光素酶表達衍生物。 Panc02 細胞源自小鼠經化學誘導產生的胰管腺癌,並被廣泛用作免疫功能正常小鼠宿主中的胰腺癌同基因模型。 導入螢光素酶報告基因,可實現體外及體內對腫瘤細胞的高靈敏度生物發光成像,從而便於對腫瘤生長、轉移擴散及治療反應進行非侵入性的縱向監測。 這些特性使 Panc02-Luc 成為胰臟癌生物學、免疫腫瘤學及臨床前藥物開發研究中極具價值的平台。

Panc02-Luc 細胞常被用於正位和小鼠皮下腫瘤模型,以研究腫瘤進展、基質相互作用、免疫細胞浸潤,以及對化療或免疫療法的耐藥機制。 由於 Panc02 腫瘤可在免疫系統完整的同基因小鼠品系中建立,因此該模型特別適用於評估檢查點抑制劑、細胞療法、癌症疫苗及聯合治療策略。基於螢光素酶的成像技術可對活體動物的腫瘤負荷進行反覆定量評估,從而降低實驗變異性,並支援治療療效的即時評估。

Panc02-Luc 細胞可用於研究胰臟腫瘤細胞的增殖、遷移、侵襲、細胞因子訊號傳導、代謝適應及凋亡。該模型的生物行為可能因工程化過程中所使用的螢光素酶構建體、啟動子系統及克隆篩選策略而有所不同。 針對特定實驗應用,進一步的特性分析數據(包括報告基因穩定性、發光強度及轉移潛能)可能至關重要。

Tissue
胰臟
Disease
小鼠胰管腺癌
Synonyms
  • Luciferase Reporter Panc02 Cell Line

02Classification

Organism
Mouse
Sex
Male
Growth properties
Adherent
GMO status
GMO-S1
GMO status details
GMO-S1: This cell line contains a stably integrated firefly luciferase reporter cassette (Luc2, codon-optimized) introduced via replication-incompetent lentiviral transduction. The resulting polyclonal cell population was maintained under puromycin selection (1–5 µg/mL). S1 containment is required. This classification applies only within Germany and may differ elsewhere.
Biosafety level
1

03Characteristics

Doubling time
24至48小時

04Identifiers

Citation
Panc02-Luc(Cytion 型號 305706)
NCBI TaxID
10090
Cellosaurus
CVCL_E3IB

05Expression and susceptibility

Antigen expression
Luc2(螢火蟲,密碼子優化)

06Handling and culture

Culture medium
RPMI 1640,含 2.0 mM 穩定谷氨醯胺,含 2.0 g/L NaHCO₃(Cytion 產品編號 820700a)
Incubation atmosphere

37°C,5% CO₂,加濕環境。

Supplements
在培養基中加入 10% 的胎牛血清(FBS)
Dissociation reagent
Accutase
Subculturing
從貼壁細胞中移除舊培養基,並用不含鈣和鎂的PBS洗滌細胞。T25培養瓶使用3-5毫升PBS,T75培養瓶則使用5-10毫升。 接著,用 Accutase 完全覆蓋細胞,T25 培養瓶使用 1–2 毫升,T75 培養瓶則使用 2.5 毫升。將細胞於室溫下孵育 8–10 分鐘,使其脫附。 孵育後,將細胞與 10 毫升培養基輕柔混合以使其重新懸浮,然後以 300xg 離心 3 分鐘。棄去上清液,將細胞重新懸浮於新鮮培養基中,並轉移至已預先加入新鮮培養基的新培養瓶中。
Seeding density
1 至 3 × 10⁴ 個細胞/cm²
Fluid renewal
每週 2 至 3 次
Thawing and culturing
  1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

  2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

  3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

  4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

  5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

  6. Centrifuge the mixture at 200 x g for 5 minutes, carefully discard the supernatant containing freezing medium.

  7. Follow the procedure described under Post-Thaw Recovery

07Quality control and cryopreservation

Freeze medium
作為冷凍保存培養基,我們使用完全生長培養基加上 10% 的二甲基亞砜(DMSO),以確保解凍後的細胞存活率達到適當水準。
Storage conditions

若需長期保存,請將試管置於溫度約為 −150 至 −196 °C 的氣相液氮中。僅在轉移至液氮之前,可將試管暫時存放於 −80 °C 環境中,作為短期過渡步驟。

Shipping conditions

冷凍保存的細胞系會以乾冰冷藏,並裝於經驗證的隔熱包裝中,包裝內含有足夠的冷媒,以確保在運輸過程中溫度維持在約 −78 °C。收到貨物後,請立即檢查容器,並盡快將試管轉移至適當的儲存場所。

08Donor

Breed / subspecies
C57BL/6
Donor age
未指定