AMO-1

The AMO-1 cell line is a multiple myeloma model derived from a malignant plasma cell neoplasm. It is extensively used in research exploring mechanisms of drug sensitivity, apoptosis, and tumor cell signaling in myeloma. AMO-1 cells are sensitive to various chemotherapeutic agents and targeted treatments, such as proteasome inhibitors and tyrosine kinase inhibitors, which have become crucial in multiple myeloma therapy. Studies have demonstrated that treatment with spleen tyrosine kinase (Syk) inhibitors induces significant anti-proliferative and pro-apoptotic effects in AMO-1 cells. This action occurs through inhibition of critical signaling pathways, including MAPK and NF-kappaB, and is characterized by increased cytochrome c release and activation of apoptotic markers like caspase-3 and PARP-1. Additionally, Syk inhibition reduces cell migration and interaction with bone marrow stromal components, further limiting myeloma cell survival.

AMO-1 cells have also shown susceptibility to natural compounds targeting the Notch3-p53 axis, such as sophocarpine derivatives. These compounds reduce cell viability by downregulating anti-apoptotic proteins (e.g., Bcl-2) and upregulating pro-apoptotic factors (e.g., Bax and cleaved caspase-3). Moreover, sophocarpine treatment decreases Notch3 expression, a pathway linked to tumor progression in myeloma and other cancers. This makes AMO-1 an effective model for testing drugs aimed at modifying Notch signaling and the apoptotic pathways mediated by p53. In immunotherapy contexts, AMO-1 cells are recognized by γδ T cells through the expression of intercellular adhesion molecule-1 (ICAM-1), and transfection to increase ICAM-1 expression enhances their susceptibility to T cell-mediated lysis. This characteristic supports studies into myeloma-targeted immunotherapies as an alternative approach to treatment-resistant cases.

37°C, 5% CO₂, humidified atmosphere.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.

To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.