CHO-STEAP1

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CHO-STEAP1 cells are recombinant Chinese hamster ovary (CHO) cells engineered to stably express human six-transmembrane epithelial antigen of the prostate 1 (STEAP1), a cell surface protein highly associated with multiple solid tumors. STEAP1 is a member of the STEAP family of metalloreductases and is characterized by six transmembrane domains with localization predominantly at the plasma membrane and intracellular vesicular compartments. Although its precise physiological function remains incompletely understood, STEAP1 has been implicated in intercellular communication, metal ion homeostasis, redox regulation, and tumor cell proliferation. Elevated STEAP1 expression has been reported in prostate cancer, Ewing sarcoma, bladder cancer, lung cancer, and several other malignancies, making it an important target in oncology-focused therapeutic development.

CHO-STEAP1 cells are widely used for development and characterization of STEAP1-targeted therapeutics, including monoclonal antibodies, antibody-drug conjugates, bispecific T-cell engagers, radioligand therapies, and engineered immune cell approaches such as CAR-T and CAR-NK therapies. The stable recombinant expression system enables quantitative analysis of antibody binding affinity, receptor occupancy, antigen density, internalization behavior, and target-specific cytotoxicity. These cells are also valuable for flow cytometry assay development, epitope mapping, high-throughput screening, and validation of STEAP1-targeted imaging agents. Because CHO cells provide a robust and relatively low-background platform for recombinant protein expression, CHO-STEAP1 models are frequently used for standardized assay development and preclinical therapeutic evaluation.

For adherent cultures: DMEM:Ham's F12 (1:1), w: 3.1 g/L Glucose, w: 2.5 mM L-Glutamine, w: 15 mM HEPES, w: 0.5 mM Sodium pyruvate, w: 1.2 g/L NaHCO3 (Cytion article number 820400a)

For suspension cultures: CHO Growth Medium A (from InSCREENeX; InSCREENeX catalog number INS-ME-1039)

37°C, 5% CO₂, humidified atmosphere.

After thawing, split the cells at a ratio of 1:2 to 1:3 in T25 flasks and allow the cells to recover from the freezing process and to adhere (for adherent cultures) for at least 24 hours.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.

To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.