RPMI 8226

RPMI 8226 cells are a human myeloma cell line that was established in 1966 from the peripheral blood of a 61-year-old male patient with multiple myeloma. This cell line was named after the Roswell Park Memorial Institute (RPMI) where it was developed, and the number 8226 denotes its specific catalog number in the cell bank.

The RPMI 8226 cell line is an important model system for studying multiple myeloma and related aspects of plasma cell biology, immunology research, and cancer therapy. RPMI 8226 cells are known to produce and secrete kappa light chains of immunoglobulins, a feature that is often exploited in research studies to investigate antibody production and secretion mechanisms.

RPMI 8226 cells exhibit numerous chromosomal abnormalities, which are typical of multiple myeloma cells. These include translocations, deletions, and amplifications that affect various oncogenes and tumor suppressor genes.

The human myeloma cell line RPMI 8226 are widely used in drug discovery and development research, and have been used to investigate drug resistance pathways and evaluating combination therapies.

In summary, RPMI 8226 cells provide a critical in vitro model for multiple myeloma research, enabling the investigation of the biological and molecular mechanisms underlying this disease and the development of therapeutic strategies.

37°C, 5% CO₂, humidified atmosphere.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.

To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.