VERO

VERO cells are widely used in developing vaccines, in the study of viral infections or malaria, and in tumor immunology and immunotherapy studies. VERO cells were derived from the kidney of an African green monkey in the 1960s by a group of Japanese scientists at Chiba University in Japan.

One of the critical characteristics of VERO cells is their rapid growth rate, with a population doubling time of approximately 24 hours. This, combined with their stability and high viral titers, makes them an ideal choice for vaccine production. As a prominent example, a Vero cell-derived vaccine for Japanese encephalitis is widely used and licensed in many countries worldwide.

Vero cells were pivotal in the development of vaccines for a plethora of infectious diseases, including the rubella virus, Ross River virus, herpes simplex virus, measles virus, and poliovirus. Vero cells are renowned for their capacity for virus production, growth, and maintenance under optimized culture conditions, making them an invaluable resource in viral vaccine production. The role of Vero cells extends to the generation of viral vectors, crucial for both vaccine development and tissue engineering applications, and virus isolation.

Different VERO cell lines, such as Vero 76 and the subclone Vero E6, offer unique characteristics suited to various research and production needs. Vero 76 cells are known for their robust growth and are widely used in vaccine production due to their high virus yield capabilities. Vero E6, on the other hand, exhibits specific properties that make it particularly useful for studying certain viruses, including enhanced sensitivity to the Ebola virus and SARS-CoV-2. This subclone's unique interaction with viruses makes it valuable for viral pathogenesis studies and antiviral drug screening.

37°C, 5% CO₂, humidified atmosphere.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.

To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.