PM-LGSOC-01

PM-LGSOC-01 is a cell line derived from peritoneal metastasis of a low-grade serous ovarian carcinoma (LGSOC). This cell line was established as part of a comprehensive research model that also included a patient-derived xenograft (PDX). The creation of PM-LGSOC-01 involved orthotopic engraftment via subperitoneal tumor slurry injection in SCID/Beige mice, leading to an early-stage transplantable peritoneal metastasis (PM)-PDX model. Histological analysis confirmed that both the PM-PDX and PM-LGSOC-01 cells retained the micropapillary and cribriform growth patterns typical of LGSOC, with tumor budding, and expression of markers such as PAX8 and WT1. Genetic analysis showed that the primary tumor, the PM, and the cell line share a KRAS c.35 G > T (p.Gly12Val) mutation, making this model relevant for studying LGSOC progression and treatment response, particularly in relation to the MAPK pathway.

PM-LGSOC-01 exhibits key characteristics relevant for preclinical research. It has a doubling time of approximately 42 hours in early passages, which decreased to 23 hours in later stages of cell culture, and has been maintained for over 100 in vitro passages. The cell line demonstrates epithelial morphology with Epithelial-like organization and high cell-cell adhesion. However, it shows limited response to platinum-based chemotherapy but is highly sensitive to paclitaxel (IC50: 6.3 ± 2.2 nM). Additionally, PM-LGSOC-01 is particularly sensitive to the MEK inhibitor trametinib (IC50: 7.2 ± 0.5 nM), both in vitro and in vivo, reflecting the impact of the KRAS mutation on therapeutic responses.

PM-LGSOC-01 serves as a valuable tool for investigating LGSOC, particularly in the context of drug resistance, tumorigenicity, and sensitivity to targeted therapies like MEK inhibitors. Its use in developing personalized treatment approaches for low-grade serous ovarian carcinoma is critical, given the poor responsiveness of LGSOC to conventional chemotherapy compared to high-grade serous ovarian carcinoma (HGSOC).

37°C, 5% CO₂, humidified atmosphere.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.

To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.