MC3T3-E1

MC3T3-E1 是一种前成骨细胞系，来源于小鼠胚胎的小腿。这些细胞被广泛用于成骨研究，特别是用于研究骨形成和分化的分子和细胞机制。MC3T3-E1 细胞系以其在体外分化成成骨细胞的强大能力而闻名，抗坏血酸和 beta-甘油磷酸酯可刺激这一过程。这种分化的标志是碱性磷酸酶、骨钙蛋白和 I 型胶原等关键成骨标志物的表达。

MC3T3-E1 细胞在骨生物学研究（包括骨基质沉积和矿化研究）中发挥着重要作用。这些细胞为研究各种药物、激素和基因修饰对成骨细胞功能和骨形成的影响提供了可靠的模型。此外，MC3T3-E1 细胞系对于研究骨质疏松症和其他骨相关疾病等病理情况也很有价值。MC3T3-E1 细胞系易于培养，对成骨刺激的反应特征明显，是研究人员揭示复杂的骨生理和病理过程的首选。

37°C, 5%_CO2, 加湿环境。

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

使用基于 PCR 的检测方法和基于发光的支原体检测方法排除支原体污染。

为确保没有细菌、真菌或酵母菌污染，每天都要对细胞培养物进行目视检查。

如需长期保存，可将样品瓶放入气相液氮中，温度约为 -150 至 -196 ℃。在-80 °C下保存只能作为转入液氮前的短暂过渡。

冷冻保存的细胞系用干冰装运，并用经过验证的隔热包装和足够的制冷剂在整个运输过程中保持约 -78 °C 的温度。收到货后，应立即检查容器，并立即将小瓶转移到适当的储藏室。

在Cytion，我们保证产品的质量和活力。 我们保证，从我们的工厂发货后 60 天内，我们的细胞在开始培养时都是存活的。 我们保证所有材料均符合分析证书和/或产品说明书中描述的规格。

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