A549

A549 cells, derived from lung adenocarcinoma tissue, are a primary model used in cancer research, particularly in biomedical laboratories focusing on lung-related cancers. A549 cells are commonly used as an in vitro model for studying lung cancer biology, drug screening, and the effects of toxic compounds.

In toxicology research, A549 cells offer a controlled experimental model that enables scientists to explore the mechanisms underlying toxic effects and cellular responses. By understanding these mechanisms, researchers can better assess the safety of substances and potentially mitigate their harmful effects.

A549 carcinoma cells have been extensively used as an in vitro model to study lung cancer pathogenesis and as an alternative tissue culture model for various pulmonary-related research studies in biomedical laboratories. These cells maintain the characteristics of type II alveolar epithelial cells and are used to examine the epithelial responses to various infections and inflammatory stimuli, including lung inflammation.

Furthermore, the human cell line A549 serves as a valuable tool in the development of specific antibodies targeting lung cancer-related proteins or markers. By exposing these cells to substances of interest, researchers can investigate how they affect cell viability, proliferation, apoptosis, and other cellular processes. This information aids in the identification of potential therapeutic targets and the development of novel treatments for lung cancer.

In summary, A549 carcinoma cells are pivotal in cancer research, especially concerning lung-related cancers, serving as an in vitro model for cancer and toxicology research, developing effective treatments, and drug screening.

37°C, 5% CO₂, humidified atmosphere.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.

To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.