CHO-K1 suspension adapted

CHO-K1 cells are a subline derived from the CHO cell line, which was originally established in the early 1950s from a Chinese hamster ovary. CHO-K1 cells are widely utilized in the production of therapeutic monoclonal antibodies and other biopharmaceuticals. Their extensive use in biopharmaceutical protein production and vaccines is attributed to their eukaryotic nature, which allows for proper folding, assembly, and post-translational modifications such as glycosylation, which influences the stability, efficacy, and safety of the produced proteins.

In the realm of recombinant protein production, the CHO-K1 cell line is used to express a wide array of proteins, including monoclonal antibodies, growth factors, cytokines, and enzymes. These proteins have applications in therapeutic treatments, diagnostic assays, and vaccine formulations.

CHO-K1 cells exhibit a robust growth rate and are adaptable to various culture conditions, including suspension and adherent cultures, making them highly valuable for large-scale bioproduction processes. They possess a high level of genetic stability and are used for stable cell line development as they are capable of amplifying and expressing exogenous genes efficiently, which is critical for producing high yields of recombinant proteins.

CHO-K1 chinese hamster cells can be easily transfected with a variety of vectors for gene expression, facillitating gene editing or knockdown. This flexibility allows researchers to introduce specific genes, silence genes, or even perform targeted gene editing using technologies like CRISPR-Cas9 in CHO-K1 host cells.

In conclusion, the chinese hamster CHO-K1 cells and CHO cells are pivotal in biotechnological research and biopharmaceutical production, offering a versatile platform for the study of gene function and the large-scale production of recombinant proteins.

37°C, 5% CO₂, humidified atmosphere. Cells must be cultivated on an orbital shaker in baffled Erlenmeyer shake flasks at 110 rpm.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.

To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.