MFC

The Mouse Forestomach Carcinoma (MFC) cell line is an invaluable tool in cancer research, particularly in the study of tumor metastasis. This cell line was established in vitro and has been subcultured for over 132 passages. MFC cells are characterized by their lack of contact inhibition and display a variety of morphologies, including round, polygonal, and spindle shapes. Ultrastructurally, MFC cells exhibit abundant microvilli on their surfaces and extensive filopodia in the cytoplasm. The nuclei of these cells are irregularly shaped with an increased nucleus-cytoplasm ratio. Additionally, desmosomes, hemidesmosomes, and a small number of tonofibrils are present.

The MFC cell line has a population doubling time of 24.7 hours, with an average mitotic index of 32.9%, reaching up to a maximum of 62% with a modal range of 70-76. The homotransplant efficiency of these cells is 100%, indicating their high viability and consistency in experimental settings. Tumors induced by MFC cells are morphologically similar to the original forestomach carcinoma from which they were derived, with 81.8% of induced tumors spontaneously metastasizing to the lungs. This high propensity for blood-borne lung metastasis makes the MFC cell line particularly useful for studying the mechanisms of tumor metastasis and for testing experimental treatments. The retention of the primary tumor's metastatic features underscores the relevance of this cell line in ongoing cancer research.

37°C, 5% CO₂, humidified atmosphere.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.

To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.