Neuro-2a

The Neuro-2a cell line, often abbreviated as N2A cells, is a mouse neuroblastoma cell line derived from the neural crest. These cells are known for their rapid proliferation and ability to differentiate into neuron-like cells under certain conditions, making them a valuable model for studying neurogenesis and neuronal differentiation. Neuro-2a cells exhibit characteristics typical of nerve cells or neuroblasts, which are precursors to fully differentiated neuronal cells.

One of the key features of mouse Neuro 2a cells is their utility in exploring the mechanisms of differentiation, particularly in the context of dopaminergic neurons. These cells can be induced to express markers characteristic of dopamine neurons, including the dopamine transporter and proteins involved in dopamine receptor localization. This makes the N2A cell line an essential tool for studies related to the normal neuroendocrine system and disorders associated with dopaminergic signaling.

The N2A cell line also provides insights into the role of various genes and proteins in neuronal function and development. For instance, the DNMT3A gene, known for its involvement in DNA methylation processes, has been studied in Neuro-2a cells to understand its impact on neuronal cells and neurodevelopmental processes. The expression of the human thyroid hormone receptor in these cells allows researchers to investigate thyroid hormone response and its influence on neurodevelopment and the differentiation of neuroblastoma cells into more mature neuronal phenotypes. Protein kinase signaling pathways are another area of intense study in N2A cells, given their critical role in mediating various cellular processes, including cell growth, differentiation, and response to extracellular signals.

In summary, the Neuro-2a (N2A) cell line, derived from mouse neuroblastoma, serves as a versatile model for studying neurogenesis, neuronal differentiation, and dopaminergic signaling, providing valuable insights into the molecular underpinnings of neurodevelopmental processes and neuroendocrine disorders.

37°C, 5% CO₂, humidified atmosphere.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.

To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.