PC3

PC3 cells, derived from the bone metastasis in a 62-year-old Caucasian male with grade IV prostatic adenocarcinoma, are a cornerstone in the study of human prostate carcinoma. The PC-3 human prostate cancer cell line is widely used for studying the molecular and cellular aspects of prostate cancer, especially in the context of metastatic disease. Their high metastatic potential makes them a valuable model for advanced prostate cancer research.

As epithelial cells, PC3 cells' lack of response to androgens and their independence from typical growth factors like glucocorticoids or fibroblast growth factors, positions them uniquely among human prostate carcinoma cells for studying the impact of koenimbin and other potential therapeutic agents.

The absence of prostate-specific antigen (PSA) expression and low activities of testosterone-5-alpha reductase and acidic phosphatase set PC3 apart from other prostate cancer cell models like LNCaP and DU145, the former known for expressing luminal differentiation markers such as AR and PSA, and the latter representing a moderated metastatic potential of prostate carcinoma.

Furthermore, the role of the PC3 prostatic carcinoma cell line in prostate cancer stem cells research is underscored by the observation that a subset forms cancer stem cell holoclones. This characteristic makes the PC3 cell line a critical model for studying the tumor environment, particularly through xenograft models where PC3 xenograft tumors are used to investigate tumor growth and response to therapies in vivo.

In summary, PC3 cells, originating from a grade IV prostatic adenocarcinoma, serve as a pivotal model in prostate cancer research due to their high metastatic potential, unique androgen independence, and distinct cellular characteristics. Their versatility extends from molecular studies of metastasis to the exploration of therapeutic responses and the investigation of prostate cancer stem cells, making them an invaluable resource for advancing our understanding of prostate carcinoma's complexities and potential treatments.

37°C, 5% CO₂, humidified atmosphere.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.

To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.