Hela 229

The HeLa 229 cell line is a clonal derivative of the original HeLa cell line, which was the first human cell line to be continuously cultured. HeLa cells were derived from cervical cancer cells taken from Henrietta Lacks in 1951. The HeLa 229 subline is utilized in various areas of biomedical research, including cancer research, drug development, and toxicology, due to its robust growth and adaptability under laboratory conditions.

One of the main characteristics of the HeLa 229 cell line is its aggressive growth and proliferation, reflecting the cancerous origin of the cells. This makes it particularly useful for studies requiring high cell yields and rapid growth, such as high-throughput screening for drug discovery. HeLa 229 cells are also highly amenable to genetic manipulation, allowing researchers to introduce foreign genes or specific mutations to study their effects on cell behavior and pathology.

HeLa 229 cells continue to be a critical model in virology, as they are susceptible to a wide variety of viruses. This susceptibility makes them an excellent tool for studying viral life cycles, host-virus interactions, and the efficacy of antiviral compounds. The cell line has also been instrumental in advancing our understanding of fundamental cellular processes, such as DNA replication, transcription, and apoptosis.

Despite their utility, the use of HeLa cells, including HeLa 229, raises ethical considerations regarding consent and the origins of the cell line, as the cells were originally obtained without the consent of Henrietta Lacks or her family. However, ongoing research with HeLa cells continues to contribute significantly to science, driven by their unique characteristics and historical importance in the development of modern cell biology.

37°C, 5% CO₂, humidified atmosphere.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.

To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.