WEHI-164

The WEHI-164 cell line was originally established from a fibrosarcoma that developed in a BALB/c mouse following subcutaneous injections of 3-methylcholanthrene. This cell line is derived from mesenchymal tissue and demonstrates characteristics typical of fibroblast-like cells. WEHI-164 has been a critical tool in the study of cancer, providing insights particularly in the fields of tumor immunology and the cellular mechanisms of apoptosis.

WEHI-164 cells are especially valued in research due to their responsiveness to cytokine-induced apoptosis, making them an important model for studying the interaction between cytokines and cancer cells. This sensitivity to cytokines like tumor necrosis factor (TNF) and TRAIL (TNF-related apoptosis-inducing ligand) positions the WEHI-164 cell line as a useful resource for exploring signaling pathways that mediate cell death and for screening potential anticancer therapies that could manipulate these pathways. Additionally, the cell line's fibroblast-like properties allow for studies on cell morphology, growth characteristics, and the tumor microenvironment, providing a more comprehensive understanding of tumor dynamics and interactions within the cellular matrix.

Despite its extensive use in research, the WEHI-164 cell line exhibits several chromosomal aberrations, which is common among cells transformed by chemical carcinogenesis. These genetic instabilities are crucial for studies focused on understanding how genetic variations can influence cancer progression and response to treatments. The ongoing use of WEHI-164 in various research setups underscores its utility in advancing the knowledge of cancer biology and in the development of novel therapeutic approaches.

37°C, 5% CO₂, humidified atmosphere.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.

To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.