C6

The C6 cell line maintains glial cell type with fibroblast morphology and originates from a glioma of a Wisthar-Furth rat. The glioma was induced by exposure to N-nitrosomethylurea, following numerous cycles of alternating culture and animal passages.

The C6 glioma cell line is frequently utilized in neuro-oncology research to create animal models that closely mimic the characteristics of human glioma, aiding in the development of new therapeutic agents and strategies. It is particularly effective in 3D cell culture and high-throughput screening.

C6 cells are genetically diverse, possessing a wild-type p53 gene, increased Rb gene expression, and a mutant p16/Cdkn2a/Ink4a locus but lacking p16 and p19ARF mRNA expression. They also overexpress several genes in human gliomas, such as PDGFβ, IGF-1, EGFR, and Erb3/Her3 precursor proteins.

However, the expression of IGF-2, FGF-9, and FGF-10 is reduced, while MMP-7 gene expression remains unchanged. Like human gliomas, C6 cells show increased activity of the Ras pathway genes, which is regulated by the elevated expression of the Ras guanine triphosphate activator protein.

The C6 cell line has been utilized in various studies. For instance, it was used to examine the ability of 2-(2,4-dihydroxy phenyl)thieno-1,3-thiazin-4-one (BChTT) to halt cancer cell proliferation and to investigate the mechanisms involved in this process.

In another research, the cytotoxic and antioxidant properties of the supercritical CO2 extract (SCE) of an old man's beard (Usnea barbata) were studied using C6 cells. Interestingly, these cells have been reported to show increased levels of glyceryl phosphate dehydrogenase activity in response to glucocorticoids.

37°C, 5% CO₂, humidified atmosphere.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.

To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.