Neuro-2a-Luc

Neuro-2a-Luc is a luciferase-expressing derivative of the Neuro-2a (N2a) mouse neuroblastoma cell line. Neuro-2a cells originate from murine neural crest-derived neuroblastoma tissue and are widely used as an in vitro model for neuronal differentiation, neurotoxicity studies, signal transduction research, and neuro-oncology investigations. Stable expression of a luciferase reporter enables sensitive, quantitative bioluminescent detection of viable cells and cellular activity, making Neuro-2a-Luc particularly useful for longitudinal monitoring in both in vitro and in vivo experimental systems. Depending on the reporter design, luciferase expression may be constitutive or linked to pathway-specific promoter activity.

Neuro-2a-Luc cells are commonly employed in applications involving tumor growth tracking, high-throughput drug screening, neural differentiation assays, and real-time assessment of therapeutic responses. In xenograft and metastasis models, luciferase-based bioluminescence imaging allows noninvasive monitoring of tumor burden and disease progression with high sensitivity. Neuro-2a-derived systems are also extensively used to study neuronal morphology, neurite outgrowth, apoptosis, oxidative stress, and mechanisms associated with neurodegenerative disease. The luciferase modification facilitates rapid quantitative analysis of cell proliferation, cytotoxicity, transcriptional activity, or pathway modulation in response to pharmacological or genetic perturbations.

As with other engineered reporter cell lines, experimental performance of Neuro-2a-Luc may depend on factors including luciferase construct integration site, promoter configuration, substrate compatibility, and stability of reporter expression over serial passage. Additional characterization data, including details regarding the luciferase variant, selection marker, and validation assays, may be required for highly specialized experimental applications.

37°C, 5% CO₂, humidified atmosphere.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 200 x g for 5 minutes, carefully discard the supernatant containing freezing medium.

7. Follow the procedure described under Post-Thaw Recovery

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.