RM-1-Luc

RM-1-Luc is a bioluminescent derivative of the murine RM-1 prostate carcinoma cell line, engineered to stably express a firefly luciferase reporter gene. The parental RM-1 cell line was established from a carcinoma of the mouse prostate gland derived from fetal C57BL/6 tissue at 17 fetal days and is a rapidly growing, androgen-independent prostate cancer model in the C57BL/6 syngeneic background. RM-1 cells exhibit epithelial morphology, express typical prostate carcinoma markers, and form aggressive tumors in immunocompetent C57BL/6 hosts, making this model suitable for studying tumor-immune interactions and evaluating immunotherapy strategies against prostate cancer.

The stable luciferase integration in RM-1-Luc enables sensitive, noninvasive bioluminescence imaging (BLI) of primary tumor growth and metastatic dissemination in living C57BL/6 mice following luciferin administration. The emitted signal correlates with viable tumor cell number, supporting longitudinal assessment of tumor engraftment, growth kinetics, and therapeutic response without repeated invasive procedures. RM-1-Luc is particularly valuable for preclinical evaluation of checkpoint inhibitors, androgen-deprivation strategies, and combination immunotherapy approaches in an immunocompetent prostate cancer model.

RM-1-Luc retains the key biological features of the parental RM-1 line, including its androgen-independent growth, C57BL/6 syngeneic compatibility, and established use in prostate cancer research. The luciferase reporter enhances experimental sensitivity and enables real-time pharmacodynamic assessment. Researchers should validate luciferase activity, growth kinetics, and immunological phenotype under their specific experimental conditions prior to large-scale in vivo use.

37°C, 5% CO₂, humidified atmosphere.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 200 x g for 5 minutes, carefully discard the supernatant containing freezing medium.

7. Follow the procedure described under Post-Thaw Recovery

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.