Caki-1

The Caki-1 cell line is derived from a metastatic site of a human renal clear cell carcinoma. Established from a tumor located in the wall of the renal vein of a male patient, Caki-1 cells are commonly used in the study of renal cancer biology, especially in understanding the mechanisms underlying clear cell renal cell carcinoma (ccRCC). This cell line is epithelial-like in morphology and exhibits robust in vitro growth characteristics, making it suitable for a variety of experimental techniques, including drug screening and molecular biology studies.

In terms of tumorigenicity, Caki-1 is capable of forming tumors in nude mice and has been reported to consistently produce clear cell carcinoma, mirroring the pathology of the renal primary tumor. This characteristic makes it an invaluable model for in vivo studies of renal cancer metastasis and tumor biology. The cell line has also been observed to metastasize to the skin in experimental settings. From a biochemical perspective, Caki-1 expresses a variety of isoenzymes and antigens, including blood type O, Rh-, and HLA types A9, B12, Bw35. Isoenzyme profiling includes AK-1, ES-D, G6PD B, GLO-I, Me-2, PGM1, and PGM3, which may be relevant in studies of cellular metabolism and genetic expression related to cancer progression and response to treatments.

37°C, 5% CO₂, humidified atmosphere.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.

To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.