SK-GT-4

The SK-GT-4 cell line is a human esophageal adenocarcinoma model derived from primary adenocarcinoma arising in dysplastic Barrett's metaplasia of the distal esophagus. This cell line is part of the SK-GT series established at the Memorial Sloan-Kettering Cancer Center and has been instrumental in studying the progression of Barrett's esophagus to esophageal adenocarcinoma. SK-GT-4 cells exhibit epithelial characteristics and serve as a valuable tool for investigating molecular mechanisms of esophageal cancer, particularly those involving p53 tumor suppressor gene mutations.

Genomic studies of SK-GT-4 have identified a mutation in the TP53 gene, specifically a G to A transition at codon 175 in exon 5, resulting in an arginine-to-histidine substitution. This mutation has been correlated with chemoresistance to DNA-damaging agents such as 5-fluorouracil, mitomycin C, and cisplatin. Studies indicate that the mutational status of p53 significantly impacts the cellular response to chemotherapy, influencing cell cycle regulation and apoptosis in SK-GT-4 cells. These features make this cell line a critical model for exploring therapeutic strategies targeting p53-associated pathways.

SK-GT-4 has been utilized to evaluate tumor invasion potential and response to growth factors in esophageal adenocarcinoma. This cell line has demonstrated invasive capabilities in Boyden chamber assays, highlighting its relevance for studying metastasis. SK-GT-4 also expresses transcripts for various growth factors, including TGF-β and platelet-derived growth factor, which contribute to its aggressive phenotype. The cell line's established role in translational research underscores its utility in both basic cancer biology and drug development.

37°C, 5% CO₂, humidified atmosphere.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.

To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.