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AH-130 FN
Tissue
肝脏
Disease
肝细胞癌
Synonyms
AH130FN-TC, AH130FN, AH-130F(N), AH-130FN, AH 130 FN

Documents

AH-130 FN

Cell line · Product number 500451

AH-130 FN is a variant of the AH-130 rat ascites tumor cell line, used extensively in studies related to coagulation, fibrinolysis, and metastasis. These ...

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01General information

Description

AH-130 FN 是 AH-130 大鼠腹水瘤细胞系的变种，广泛用于凝血、纤溶和转移相关研究。这些细胞来源于大鼠，通常通过连续腹腔植入雄性唐柳大鼠来维持。AH-130 细胞系本身以其高度的血栓形成活性和纤维蛋白溶解活性而闻名，这与其在促进血液转移（尤其是肺部转移）方面的作用有关。相比之下，AH-130 FN 变体的血栓形成活性和纤维蛋白溶解活性较低。AH-130 和 AH-130 FN 在酶活性上的这种差异至关重要，因为它影响着静脉注射后血栓的形成和肺部转移灶的数量。

研究表明，静脉注射后，AH-130 细胞会导致血小板数量和纤维蛋白原水平显著下降，表明血栓形成增加。与 AH-130 FN 相比，这种效果更为明显。组织学研究表明，与 AH-130 FN 相比，AH-130 在接种后 72 小时和 7 天内在肺部形成的转移灶更多。AH-130 与栓塞肿瘤细胞周围由血小板和纤维蛋白组成的血栓形成有关，而 AH-130 FN 则显示血栓形成稀少。这些发现表明，AH-130 较高的血栓形成活性在通过血小板聚集和纤维蛋白沉积在肿瘤细胞周围促进转移方面发挥了重要作用，而 AH-130 FN 的这一过程则不那么突出。

Tissue
肝脏
Disease
肝细胞癌
Synonyms
  • AH130FN-TC
  • AH130FN
  • AH-130F(N)
  • AH-130FN
  • AH 130 FN

02Classification

Organism
Rat
Growth properties
Adherent and suspension
Biosafety level
1

03Characteristics

Morphology
悬浮液中的圆形细胞，粘附时呈上皮样
Tumourigenic
是的，在 Wistar 大鼠身上。

04Identifiers

Citation
AH-130 FN（Cytion 目录号 500451）
NCBI TaxID
10116
Cellosaurus
CVCL_5683

05Expression and susceptibility

Viruses
RAP 测试阴性。.

06Handling and culture

Culture medium
DMEM:Ham's F12（1:1），w: 3.1 g/L 葡萄糖，w: 2.5 mM L-谷氨酰胺，w: 15 mM HEPES，w: 0.5 mM 丙酮酸钠，w: 1.2 g/L NaHCO3（Cytion 文章编号 820400a）。
Incubation atmosphere

37°C, 5%CO2, 加湿环境。

Supplements
在培养基中添加 10% FBS
Subculturing
通过上下吸取操作轻柔匀化烧瓶中的细胞悬液，随后取代表性样本测定每毫升细胞密度。用新鲜培养基将悬液稀释至1×10⁵细胞/毫升浓度，并将调整后的悬液分装至新烧瓶中进行后续培养。
Seeding density
1 × 10⁶ 个细胞/平方厘米
Fluid renewal
每 3 至 5 天
Post-thaw recovery
解冻后，将细胞以5×10⁴个细胞/平方厘米的密度接种于培养皿中，使细胞从冷冻过程中恢复并至少贴壁24小时。
Thawing and culturing

  1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

  2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

  3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

  4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

  5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

  6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

  7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

  8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

07Quality control and cryopreservation

Sterility

使用基于 PCR 的检测方法和基于发光的支原体检测方法排除支原体污染。

为确保没有细菌、真菌或酵母菌污染，每天都要对细胞培养物进行目视检查。

Freeze medium
我们使用完全生长培养基（包括 FBS）+10% DMSO 或 CM-1（Cytion 商品目录编号 800100）作为冷冻保存培养基，以获得足够的解冻后存活率，CM-1（Cytion 商品目录编号 800100）含有优化的渗透保护剂和代谢稳定剂，可提高恢复能力并减少冷冻引起的应激。
Storage conditions

如需长期保存，可将样品瓶放入气相液氮中，温度约为 -150 至 -196 ℃。在-80 °C下保存只能作为转入液氮前的短暂过渡。

Shipping conditions

冷冻保存的细胞系用干冰装运，并用经过验证的隔热包装和足够的制冷剂在整个运输过程中保持约 -78 °C 的温度。收到货后，应立即检查容器，并立即将小瓶转移到适当的储藏室。