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PSN-1
Tissue
胰腺
Disease
胰腺腺癌
Synonyms
PSN1

Documents

PSN-1

Cell line · Product number 305618

The PSN-1 cell line is a human pancreatic adenocarcinoma model established from a primary pancreatic tumor. It is particularly notable for harboring ampli...

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Recommended (freshest lot)
  • CoA
1

01Description

02General information

Description

PSN-1细胞系是一种由原发性胰腺肿瘤建立的人胰腺腺癌模型。 该细胞系的一个特别显著特征是，它同时携带 c-myc 致癌基因的扩增和 c-Ki-ras 基因的扩增，且后者在第 12 密码子处存在点突变。该突变将甘氨酸替换为精氨酸（GGT 变为 CGT），从而产生了一种具有转化潜能的 c-Ki-ras 激活形式。 PSN-1 保留了与原始肿瘤组织相同的分子特征，包括 c-myc 基因 50 倍的扩增以及突变型 c-Ki-ras 基因 3 至 6 倍的扩增。这些致癌基因的扩增伴随着两个基因 mRNA 表达的显著增加，表明它们在肿瘤进展中起着关键作用。

PSN-1是通过一种非常规过程衍生的：将未添加冷冻保护剂、在-80°C下保存了1.5年的冷冻肿瘤组织成功移植到裸鼠体内。 从由此形成的异种移植瘤中，建立了 PSN-1 细胞系，并在体外显示出稳定的致癌基因扩增，与原始肿瘤及其转移性淋巴结中发现的情况一致。 该细胞系表现出异质性生长特征，细胞既可作为单层细胞强力附着，也可在悬浮液中生长。这种双重行为反映了细胞群的多样性，能够适应不同的培养条件。

Tissue
胰腺
Disease
胰腺腺癌
Applications
三维细胞培养
Synonyms
  • PSN1

03Classification

Organism
Human
Sex
Male
Growth properties
Adherent
Biosafety level
1

04Characteristics

Morphology
上皮样
Cell type
上皮细胞
Doubling time
21 小时

05Identifiers

Citation
PSN-1（Cytion 产品编号 305618）
NCBI TaxID
9606
Cellosaurus
CVCL_1644

06Expression and susceptibility

Mutational profile
基因缺失：CDKN2A，纯合子；基因缺失：SMAD4，纯合子；突变：KRAS，简单突变，p.Gly12Arg (c.34G>C)，杂合子； 突变：TP53，简单突变，p.Lys132Gln (c.394A>C)，纯合子

07Handling and culture

Culture medium
RPMI 1640，w：2.0 mM 稳定谷氨酰胺，w：2.0 g/L NaHCO3（Cytion 文章编号 820700a）。
Incubation atmosphere

37°C, 5%CO2, 加湿环境。

Supplements
在培养基中添加 10% 热灭活 FBS
Dissociation reagent
Accutase
Fluid renewal
每周 2 至 3 次
Thawing and culturing

  1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

  2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

  3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

  4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

  5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

  6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

  7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

  8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

08Quality control and cryopreservation

Sterility

使用基于 PCR 的检测方法和基于发光的支原体检测方法排除支原体污染。

为确保没有细菌、真菌或酵母菌污染，每天都要对细胞培养物进行目视检查。

Freeze medium
我们使用完全生长培养基（包括 FBS）+10% DMSO 或 CM-1（Cytion 商品目录编号 800100）作为冷冻保存培养基，以获得足够的解冻后存活率，CM-1（Cytion 商品目录编号 800100）含有优化的渗透保护剂和代谢稳定剂，可提高恢复能力并减少冷冻引起的应激。
Storage conditions

如需长期保存，可将样品瓶放入气相液氮中，温度约为 -150 至 -196 ℃。在-80 °C下保存只能作为转入液氮前的短暂过渡。

Shipping conditions

冷冻保存的细胞系用干冰装运，并用经过验证的隔热包装和足够的制冷剂在整个运输过程中保持约 -78 °C 的温度。收到货后，应立即检查容器，并立即将小瓶转移到适当的储藏室。

09Donor

Donor age
未说明
Ethnicity
东亚