PSN-1

01Description

02General information

Description

The PSN-1 cell line is a human pancreatic adenocarcinoma model established from a primary pancreatic tumor. It is particularly notable for harboring amplifications of both the c-myc oncogene and the c-Ki-ras gene with a point mutation at codon 12. This mutation substitutes arginine for glycine (GGT to CGT), leading to an activated form of c-Ki-ras with transforming potential. PSN-1 retains the same molecular characteristics as the original tumor tissue, including a 50-fold amplification of c-myc and a 3- to 6-fold amplification of the mutated c-Ki-ras. These oncogenic amplifications are accompanied by significantly increased mRNA expression for both genes, suggesting their critical role in tumor progression.

PSN-1 was derived through an unconventional process where frozen tumor tissues, stored for 1.5 years at -80°C without cryopreservation agents, were successfully transplanted into nude mice. From the resulting xenograft, the PSN-1 cell line was established and demonstrated stable oncogenic amplifications in vitro, mirroring those found in the original tumor and its metastatic lymph nodes. The cell line displays heterogeneous growth characteristics, with cells either adhering strongly as monolayers or growing in suspension. This dual behavior reflects a diverse cell population capable of adapting to different culture conditions.

Tissue

Pancreas

Disease

Pancreatic adenocarcinoma

Applications

3D cell culture

Synonyms

• PSN1

03Classification

Organism

Human

Sex

Male

Growth properties

Adherent

Biosafety level

1

04Characteristics

Morphology

Epithelial-like

Cell type

Epithelial cell

Doubling time

21 hours

05Identifiers

Citation

PSN-1 (Cytion catalog number 305618)

NCBI TaxID

9606

Cellosaurus

CVCL_1644

06Expression and susceptibility

Mutational profile

Gene deletion: CDKN2A, Homozygous; Gene deletion: SMAD4, Homozygous; Mutation: KRAS, Simple, p.Gly12Arg (c.34G>C), Heterozygous; Mutation: TP53, Simple, p.Lys132Gln (c.394A>C), Homozygous

07Handling and culture

Culture medium

RPMI 1640, w: 2.0 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a)

Incubation atmosphere

37°C, 5% CO₂, humidified atmosphere.

Supplements

Supplement the medium with 10% FBS

Dissociation reagent

Accutase

Fluid renewal

2 to 3 times per week

Thawing and culturing

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

08Quality control and cryopreservation

Sterility

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.

To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

Freeze medium

As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.

Storage conditions

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Shipping conditions

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

09Donor

Donor age

Unspecified

Ethnicity

East Asian

10Legal and safety

Safety precautions

When planning to store a cryovial in liquid nitrogen for future thawing, it is mandatory to adhere to stringent safety measures. Appropriate protective gloves and clothing are essential, and the use of a face mask or safety goggles is required during the transfer of frozen samples to or from the liquid nitrogen tank. This is to mitigate the risk of injury from potential cryovial explosions upon removal, which can result in the projection of sharp fragments.

Warranty

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.

Material Transfer Agreement required

Yes