HK EGFP-H2B

The HK EGFP-H2B cell line is a genetically modified Hela Kyoto cell line used primarily for the study of chromatin dynamics and nuclear processes. This cell line expresses a fusion protein consisting of Enhanced Green Fluorescent Protein (EGFP) and histone H2B. The integration of EGFP into the H2B protein allows for the real-time visualization of chromatin in living cells under fluorescence microscopy, providing valuable insights into the spatial and temporal organization of the nucleus.

The EGFP-H2B fusion facilitates numerous applications in cell biology, including the study of cell cycle progression, mitosis, and gene expression regulation. By observing the fluorescence patterns, researchers can identify and analyze phases of the cell cycle, chromosomal segregation, and structural changes within the nucleus. This cell line is derived from adult human cells, ensuring relevance to human biology, and is utilized in both basic biological research and more applied pharmaceutical studies.

Additionally, the HK EGFP-H2B cell line serves as a crucial tool in epigenetics research. The ability to directly observe histone behaviors helps in understanding the epigenetic mechanisms that underlie gene expression and silencing, as well as the effects of various epigenetic modifiers. The cell line's robust application in live-cell imaging experiments makes it indispensable for detailed studies requiring dynamic cellular analysis.

37°C, 5% CO₂, humidified atmosphere.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.

To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.