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MH7A
Tissue
滑膜
Disease
滑膜成纤维细胞（SV40永生化；模拟类风湿性关节炎滑膜细胞）
Metastatic site
不适用（非致瘤性滑膜成纤维细胞模型）

Documents

MH7A

Cell line · Product number 305036

MH7A is a human synovial fibroblast cell line immortalized by stable SV40 large T antigen expression, established from synovial tissue of a female Japanes...

US$800.00excl. tax and shipping
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Recommended (freshest lot)
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1

01General information

Description

MH7A 是一种通过稳定表达 SV40 大 T 抗原而实现永生化的重组人滑膜成纤维细胞系，源自一名日本女性患者的滑膜组织。 MH7A 以贴壁培养方式生长，具有成纤维细胞样形态，并保留了类风湿性关节炎滑膜成纤维细胞（RASFs）的关键功能特征，包括对促炎性细胞因子（IL-1β、 TNF-α）的反应性、基质金属蛋白酶（MMPs）的表达以及侵袭软骨基质的能力。它是研究滑膜细胞生物学和类风湿性关节炎发病机制最广泛使用的体外模型之一。

MH7A 适用于类风湿性关节炎研究、滑膜细胞活化、MMP 和 TIMP 调节、细胞因子信号传导（NF-κB、JAK-STAT、MAPK）、抗风湿药物评估（DMARDs、生物制剂、JAK 抑制剂）以及关节破坏的共培养模型。 该细胞系提供了一种一致且可重复的类风湿性关节炎滑膜细胞模型，从而规避了源自患者的一级类风湿性关节炎滑膜成纤维细胞（RASFs）所存在的不稳定性及供应有限的问题。

Tissue
滑膜
Disease
滑膜成纤维细胞（SV40永生化；模拟类风湿性关节炎滑膜细胞）
Metastatic site
不适用（非致瘤性滑膜成纤维细胞模型）
Applications
类风湿性关节炎研究；滑膜细胞活化；MMP/TIMP调节；NF-κB/JAK-STAT/MAPK信号传导；抗风湿药物评价；关节破坏共培养模型；细胞因子信号传导

02Classification

Organism
Human
Sex
Female
Growth properties
Adherent
GMO status
GMO-S1
GMO status details
GMO-S1: MH7A was immortalized by stable integration of SV40 large T antigen. This classification applies only within Germany and may differ elsewhere.
Biosafety level
1

03Characteristics

Morphology
成纤维细胞样
Cell type
成纤维细胞样滑膜细胞
Doubling time
约24至36小时

04Identifiers

Citation
MH7A（Cytion 产品编号 305036）
NCBI TaxID
9606
Cellosaurus
CVCL_0427

05Handling and culture

Culture medium
RPMI 1640，w：2.0 mM 稳定谷氨酰胺，w：2.0 g/L NaHCO3（Cytion 文章编号 820700a）。
Incubation atmosphere

37°C, 5%CO2, 加湿环境。

Supplements
在培养基中添加 10% FBS
Dissociation reagent
Accutase，5分钟，37°C
Subculturing
移除培养基，用不含钙和镁的PBS冲洗，加入0.25%胰蛋白酶浸没细胞，在37°C下孵育3–5分钟，用培养基重悬细胞，以300×g离心3分钟，弃去上清液，重新接种。
Split ratio
1 : 8 的比例
Seeding density
1 至 3 × 10⁴ 个细胞/cm²
Fluid renewal
每周 2 至 3 次
Post-thaw recovery
解冻后，将细胞以2至5×10⁴个细胞/平方厘米的密度接种于培养皿中，使细胞从冷冻过程中恢复并至少贴壁24小时。
Thawing and culturing

  1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

  2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

  3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

  4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

  5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

  6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

  7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

  8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

06Quality control and cryopreservation

Freeze medium
我们使用完全生长培养基（包括 FBS）+10% DMSO 或 CM-1（Cytion 商品目录编号 800100）作为冷冻保存培养基，以获得足够的解冻后存活率，CM-1（Cytion 商品目录编号 800100）含有优化的渗透保护剂和代谢稳定剂，可提高恢复能力并减少冷冻引起的应激。
Storage conditions

如需长期保存，可将样品瓶放入气相液氮中，温度约为 -150 至 -196 ℃。在-80 °C下保存只能作为转入液氮前的短暂过渡。

Shipping conditions

冷冻保存的细胞系用干冰装运，并用经过验证的隔热包装和足够的制冷剂在整个运输过程中保持约 -78 °C 的温度。收到货后，应立即检查容器，并立即将小瓶转移到适当的储藏室。

07Donor

Donor age
年龄不详
Ethnicity
日语