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SV-80
Tissue
皮肤
Disease
正常皮肤成纤维细胞（SV40永生化；无致瘤性）
Metastatic site
不适用（正常成纤维细胞系；非肿瘤样本）
Synonyms
SV-80, SV 80, SV-A clone 80, SV clone 80, Simian virus 80

Documents

SV-80

Cell line · Product number 300345

This SV40-transformed line was originally generated using cells which were derived from a skin biopsy of an adult female (strain A) by Todaro et al. in 1963,...

US$395.00excl. tax and shipping
In stock
Lot
Recommended (freshest lot)
  • CoA
  • CoA
1

01General information

Description
这种 SV40 转基因品系最初是由 Todaro 等人于 1963 年从一名成年女性（A 株）的皮肤活检组织中提取的细胞生成的，而不是从一名五个月大的男性胎儿（C 株）的肺组织中提取的细胞生成的。感染后，生长的菌落形态发生了变化，产生了成纤维细胞型和上皮细胞型菌落。因此，将 SV-80 定义为肺源性细胞并保留下来很可能是无效的。不过，该细胞系将在 p53 抗原和大 T 抗原的存在方面进一步定性。
Tissue
皮肤
Disease
正常皮肤成纤维细胞（SV40永生化；无致瘤性）
Metastatic site
不适用（正常成纤维细胞系；非肿瘤样本）
Applications
DNA修复研究；SV40永生化成纤维细胞生物学；细胞遗传学；基因毒性检测；用于癌症对照研究的正常人成纤维细胞参考样本；SV40大T抗原生物学
Synonyms
  • SV-80
  • SV 80
  • SV-A clone 80
  • SV clone 80
  • Simian virus 80

02Classification

Organism
Human
Sex
Female
Growth properties
Adherent
GMO status
GMO-S1
GMO status details
GMO-S1: This SV-80 human fibroblast line contains SV40 T-antigen sequences enabling immortalization for DNA repair and cytogenetics research. This classification applies only within Germany and may differ elsewhere.
Biosafety level
1

03Characteristics

Morphology
上皮样
Cell type
成纤维细胞
Tumourigenic
SMRV：阴性，经实时 PCR 验证
Karyotype
模数 = 76，范围 = 52 至 87
Doubling time
20 至 24 小时

04Identifiers

Citation
SV-80 （Cytion 目录号 300345）
NCBI TaxID
9606
Cellosaurus
CVCL_0541

05Handling and culture

Culture medium
DMEM，w：4.5 克/升葡萄糖，w：4 毫摩尔 L-谷氨酰胺，w：3.7 克/升 NaHCO3，w：1.0 毫摩尔丙酮酸钠（Cytion 编号 820300a）。
Incubation atmosphere

37°C, 5%CO2, 加湿环境。

Supplements
在培养基中添加 10% FBS
Dissociation reagent
Accutase
Subculturing
去除粘附细胞上的旧培养基，用不含钙和镁的 PBS 冲洗。T25 烧瓶用 3-5 毫升 PBS，T75 烧瓶用 5-10 毫升。然后用 Accutase 完全覆盖细胞，T25 烧瓶用 1-2 毫升，T75 烧瓶用 2.5 毫升。让细胞在室温下孵育 8-10 分钟，使其分离。孵育后，用 10 毫升培养基轻轻混合细胞使其重悬，然后用 300xg 离心 3 分钟。弃去上清液，用新鲜培养基重悬细胞，并将其转移到已装有新鲜培养基的新烧瓶中。
Split ratio
1 到 5
Seeding density
3 至 5 × 10³ 个细胞/cm²
Fluid renewal
每周 1 至 2 次
Post-thaw recovery
快速
Thawing and culturing

  1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

  2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

  3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

  4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

  5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

  6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

  7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

  8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

06Quality control and cryopreservation

Sterility

使用基于 PCR 的检测方法和基于发光的支原体检测方法排除支原体污染。

为确保没有细菌、真菌或酵母菌污染，每天都要对细胞培养物进行目视检查。

Freeze medium
我们使用完全生长培养基（包括 FBS）+10% DMSO 或 CM-1（Cytion 商品目录编号 800100）作为冷冻保存培养基，以获得足够的解冻后存活率，CM-1（Cytion 商品目录编号 800100）含有优化的渗透保护剂和代谢稳定剂，可提高恢复能力并减少冷冻引起的应激。
Storage conditions

如需长期保存，可将样品瓶放入气相液氮中，温度约为 -150 至 -196 ℃。在-80 °C下保存只能作为转入液氮前的短暂过渡。

Shipping conditions

冷冻保存的细胞系用干冰装运，并用经过验证的隔热包装和足够的制冷剂在整个运输过程中保持约 -78 °C 的温度。收到货后，应立即检查容器，并立即将小瓶转移到适当的储藏室。

07Donor

Donor age
成人
Ethnicity
高加索人