ProPak-A.6

ProPak-A.6 (PPA.6) is a human fetal kidney-derived packaging cell line generated for the production of replication-incompetent retroviral vectors. ProPak-A.6 stably expresses adenovirus-5 E1A sequences (for transcriptional transactivation) together with gag-, pol-, and env-related proteins derived from murine leukemia virus (MLV), enabling efficient packaging of recombinant retroviral genomes into non-infectious MLV-based viral particles. The GMO-S1 classification reflects the stable integration of these viral transgenes.

ProPak-A.6 is applicable as a packaging cell line for generating high-titer recombinant MLV-based retroviral vectors for gene transfer into target cells. It is used in gene therapy vector production, stable transduction of dividing cell populations, and studies requiring efficient retroviral delivery of transgenes. The adenovirus E1A component enhances promoter-driven expression, improving viral production efficiency. Users should follow applicable biosafety regulations for BSL-2 retroviral vector work.

37°C, 5% CO₂, humidified atmosphere.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 200 x g for 5 minutes, carefully discard the supernatant containing freezing medium.

7. Follow the procedure described under Post-Thaw Recovery

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.