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H4-II-E-C3
Tissue
肝脏
Disease
肝细胞癌
Synonyms
H4IIEC3, H4-IIE-C3, H4-II-EC3, H-II-E-C3

Documents

H4-II-E-C3

Cell line · Product number 305336

The H4-II-E-C3 cell line is a well-established rat hepatoma cell line derived from liver carcinoma. This line is widely used in research focused on liver-...

US$540.00excl. tax and shipping
In stock
Lot
Recommended (freshest lot)
  • CoA
1

01General information

Description

H4-II-E-C3细胞系是一种源自肝癌的、已建立成熟的大鼠肝癌细胞系。该细胞系广泛应用于针对肝脏特异性功能以及肝细胞内代谢过程的研究。 H4-II-E-C3 细胞的一个显著特点是其高亲和力谷氨酸转运蛋白 GLT-1A 的高表达。这一特征使其区别于正常大鼠肝细胞，后者并不表现出该转运蛋白的显著表达水平。 H4-II-E-C3细胞中GLT-1A的表达使得对谷氨酸代谢和转运的研究变得高效，从而为肝细胞转化和肿瘤细胞代谢提供了新的见解。

此外，H4-II-E-C3细胞还被用于研究醛类代谢，特别是线粒体Ⅱ类醛脱氢酶（ALDH2）的活性。该酶对代谢乙醇氧化过程中产生的乙醛至关重要，因此该细胞系适用于酒精代谢及其细胞效应的研究。 在这些细胞中采用“乙醛钳”技术，无需分离线粒体即可精确测定ALDH2活性，从而促进了酶动力学研究以及各种抑制剂对醛类解毒影响的研究。

Tissue
肝脏
Disease
肝细胞癌
Synonyms
  • H4IIEC3
  • H4-IIE-C3
  • H4-II-EC3
  • H-II-E-C3

02Classification

Organism
Rat
Sex
Male
Growth properties
Adherent
GMO status
GMO-S1
GMO status details
GMO-S1: This rat hepatocellular carcinoma cell line (H4-II-E-C3) contains undefined modifications classified as GMO, stably present in the transformed hepatic cell line. This classification applies only within Germany and may differ elsewhere.
Biosafety level
1

03Characteristics

Tumourigenic
是的，在AxC大鼠中

04Identifiers

Citation
H4-II-E-C3（Cytion 产品编号 305336）
NCBI TaxID
10116
Cellosaurus
CVCL_0285

05Expression and susceptibility

Protein expression
基因表达：酪氨酸氨基转移酶、白蛋白、转铁蛋白、凝血酶原

06Handling and culture

Culture medium
DMEM，w：4.5 克/升葡萄糖，w：4 毫摩尔 L-谷氨酰胺，w：3.7 克/升 NaHCO3，w：1.0 毫摩尔丙酮酸钠（Cytion 编号 820300a）。
Incubation atmosphere

37°C, 5%CO2, 加湿环境。

Supplements
在培养基中添加 10% FBS
Dissociation reagent
Accutase
Subculturing
去除粘附细胞上的旧培养基，用不含钙和镁的 PBS 冲洗。T25 烧瓶用 3-5 毫升 PBS，T75 烧瓶用 5-10 毫升。然后用 Accutase 完全覆盖细胞，T25 烧瓶用 1-2 毫升，T75 烧瓶用 2.5 毫升。让细胞在室温下孵育 8-10 分钟，使其分离。孵育后，用 10 毫升培养基轻轻混合细胞使其重悬，然后用 300xg 离心 3 分钟。弃去上清液，用新鲜培养基重悬细胞，并将其转移到已装有新鲜培养基的新烧瓶中。
Seeding density
1-3×10⁴/cm²
Fluid renewal
每周 2 至 3 次
Thawing and culturing

  1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

  2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

  3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

  4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

  5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

  6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

  7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

  8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

07Quality control and cryopreservation

Sterility

使用基于 PCR 的检测方法和基于发光的支原体检测方法排除支原体污染。

为确保没有细菌、真菌或酵母菌污染，每天都要对细胞培养物进行目视检查。

Freeze medium
我们使用完全生长培养基（包括 FBS）+10% DMSO 或 CM-1（Cytion 商品目录编号 800100）作为冷冻保存培养基，以获得足够的解冻后存活率，CM-1（Cytion 商品目录编号 800100）含有优化的渗透保护剂和代谢稳定剂，可提高恢复能力并减少冷冻引起的应激。
Storage conditions

如需长期保存，可将样品瓶放入气相液氮中，温度约为 -150 至 -196 ℃。在-80 °C下保存只能作为转入液氮前的短暂过渡。

Shipping conditions

冷冻保存的细胞系用干冰装运，并用经过验证的隔热包装和足够的制冷剂在整个运输过程中保持约 -78 °C 的温度。收到货后，应立即检查容器，并立即将小瓶转移到适当的储藏室。

08Donor

Breed / subspecies
AxC
Donor age
未说明