CCK-81

The CCK-81 cell line is a human colorectal adenocarcinoma model derived from a primary tumor. It is commonly used in cancer biology studies focusing on gastrointestinal malignancies and has been characterized for various genetic alterations and drug response profiles. According to functional screening of tumor suppressor genes, CCK-81 expresses mutant **TP53**, as confirmed by yeast-based functional assays, with only about 6% of colonies showing transcriptionally active p53 phenotype, indicating a loss-of-function mutation. This mutation status aligns with its tumorigenic origin and contributes to its relevance as a model for studying p53-deficient colorectal cancers.

CCK-81 has also been included in major cancer cell line compendia such as the Cancer Cell Line Encyclopedia (CCLE), where it has been profiled across multiple omics layers including gene expression, copy number variation, mutation status, and drug sensitivity. Its inclusion in these datasets allows for integrated analyses of pathway dependencies and therapeutic vulnerabilities across colorectal cancer subtypes. For instance, proteogenomic profiling has highlighted that colorectal cancer cell lines, including CCK-81, often exhibit dysregulated signaling pathways such as Wnt/β-catenin and MAPK, making them suitable for precision oncology studies targeting these axes.

37°C, 5% CO₂, humidified atmosphere.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.

To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.