L929-GFP

L-929-GFP cells are a fluorescently labeled derivative of the murine L-929 fibroblast cell line, which was originally established from subcutaneous connective tissue of an adult mouse. The parental L-929 line is one of the most extensively used mouse fibroblast models in biomedical research and is characterized by its adherent growth, spindle-shaped morphology, and robust proliferative capacity. L-929 cells are widely utilized in studies of cytotoxicity, inflammation, extracellular matrix biology, and host–pathogen interactions, and they are also commonly employed for the production and bioassay of cytokines such as tumor necrosis factor-α (TNF-α).

The stable expression of green fluorescent protein (GFP) in L-929-GFP cells enables direct visualization and quantitative tracking of fibroblast behavior in real time. These cells are particularly useful for fluorescence-based applications including migration assays, co-culture experiments, tissue engineering studies, and live-cell imaging. L-929-GFP cells retain the core biological characteristics of the parental fibroblast line while providing enhanced utility for monitoring cell localization, proliferation, and interactions within complex cellular environments. Consequently, they serve as a versatile model for investigating stromal cell dynamics, wound healing processes, biomaterial compatibility, and immune-mediated cytotoxic responses.

Genetic modification: Stably modified by replication-incompetent lentiviral transduction to express the ZsGreen1 green fluorescent protein reporter; maintained as a polyclonal population under puromycin selection (1–5 µg/mL). S1/BSL-1 containment.

37°C, 5% CO₂, humidified atmosphere.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 200 x g for 5 minutes, carefully discard the supernatant containing freezing medium.

7. Follow the procedure described under Post-Thaw Recovery

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.