L Wnt-3A

The L Wnt-3A cell line is a derivative of the L cells, originally derived from mouse fibroblast cells. This cell line is specifically engineered to stably express the Wnt-3A protein, a critical component of the Wnt signaling pathway. Wnt signaling is crucial for various developmental processes, including cell proliferation, differentiation, and migration. The stable expression of Wnt-3A in this cell line makes it a valuable tool for studying the molecular mechanisms underlying these biological processes, particularly in the context of cancer research, tissue regeneration, and embryonic development.

Researchers often utilize the L Wnt-3A cell line to produce conditioned medium rich in Wnt-3A, which can then be used to activate Wnt signaling in other cell types. This application is especially beneficial in the study of stem cell biology and regenerative medicine, where Wnt signaling plays a pivotal role in maintaining stem cell pluripotency and promoting tissue repair. Additionally, the cell line serves as a model to investigate the dysregulation of Wnt signaling in various cancers, providing insights into potential therapeutic targets and treatments.

Due to the robust and reliable expression of Wnt-3A, the L Wnt-3A cell line is widely used in laboratories to explore the effects of Wnt signaling on different cellular processes. It is an indispensable resource for scientists aiming to unravel the complexities of Wnt-mediated cellular functions and to develop novel strategies for modulating this pathway in disease contexts.

37°C, 5% CO₂, humidified atmosphere.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.

To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.