HL-1

HL-1 is a cardiac muscle cell line derived from the atrial cardiomyocytes of an adult mouse. This cell line is unique in that it retains the ability to contract and maintain a differentiated cardiac phenotype over long-term culture, making it an invaluable model for cardiovascular research. HL-1 cells are widely used in studies focused on cardiac physiology, electrophysiology, and pharmacology. Their ability to undergo spontaneous and inducible contractions allows researchers to investigate the molecular mechanisms underlying cardiac muscle function, disease, and response to therapeutic agents.

HL-1 cells exhibit characteristics typical of cardiac muscle cells, including the expression of cardiac-specific markers such as troponin, myosin, and connexin 43. They are responsive to various physiological and pharmacological stimuli, enabling detailed studies of cardiac signal transduction pathways, ion channel function, and the effects of drugs on heart cells. This cell line is particularly valuable for modeling cardiac arrhythmias, hypertrophy, and other cardiac pathologies. Additionally, HL-1 cells are used in high-throughput drug screening assays aimed at identifying compounds that modulate cardiac function, which is crucial for the development of new treatments for cardiovascular diseases.

Claycomb medium, 2 mM glutamine, 10% FBS, 0.1 mM norepinephrine in L-ascorbic acid (We do not sell)

Prepare 0.1 mM norepinephrine in L-ascorbic acid:

Prepare a 100-fold stock solution = 10 mM norepinephrine.

1. Prepare a 30 mM L-ascorbic acid solution (50 mL)

2. Add 160 mg of norepinephrine to the ascorbic acid solution (50 mL) = 10 mM norepinephrine

3. Sterilely filter the solution through a 0.2-µm filter cartridge.

4. Fill 1-mL aliquots of the 100-fold norepinephrine stock solution into sterile vials and store at −20 °C. Wrap in aluminum foil; must be protected from light. The solution is stable for approximately 1 month at −20 °C.

5. Add 1 mL of this solution to 100 mL of Claycomb medium. The medium is stable for only 1 month.

37°C, 5% CO₂, humidified atmosphere.

The cell culture bottles must be coated with fibronectin and gelatin. 25 µg fibronectin, dissolved in 2 ml of 0.02% gelatin in water, are placed in a T25 and incubated overnight at 37°C

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.

To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.