Lenti-X293T

Lenti-X293T cells are a derivative of the human embryonic kidney 293T lineage, engineered and optimized specifically for high-efficiency lentiviral vector production. Like parental 293T cells, they stably express the SV40 large T antigen, which enables episomal replication of plasmids containing the SV40 origin of replication and significantly enhances transient transfection efficiency. Lenti-X293T cells exhibit an adherent epithelial morphology and robust growth characteristics in standard serum-supplemented culture conditions, supporting high-density cultures suitable for large-scale viral production workflows.

This cell line has been selected for superior transfection performance using calcium phosphate, lipid-based, or polymer-based reagents, resulting in consistently elevated lentiviral titers compared to conventional HEK293T populations. The enhanced viral output is attributed to optimized cellular physiology that supports efficient plasmid uptake, strong transgene expression, and effective assembly and release of replication-incompetent lentiviral particles when co-transfected with appropriate packaging and envelope constructs. Lenti-X293T cells are therefore widely used for the generation of third-generation lentiviral vectors in gene delivery, gene editing, and stable cell line engineering applications.

Lenti-X293T cells maintain the general utility of HEK293-derived systems for high-level recombinant protein expression and transient gene expression studies. Their stable growth characteristics and reproducible performance make them suitable for both small-scale research applications and scalable production settings, provided that standard biosafety and vector packaging guidelines are followed for lentiviral systems.

37°C, 5% CO₂, humidified atmosphere.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 200 x g for 5 minutes, carefully discard the supernatant containing freezing medium.

7. Follow the procedure described under Post-Thaw Recovery

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.